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The Importance of Testing (Free) Testosterone Levels in Women and Men and How to Increase Your Testosterone Level Naturally

Happy Couple with Optimal Testosterone Levels

Testosterone is an important steroid hormone that plays many functional roles in your body; including the orchestration of metabolism; energy production, and use; the maintenance of skeletal and body protein; the integrity and development of cognitive abilities and behaviors; control of the reproductive system; and more.1

Research shows boosting low testosterone levels into the normal range improves overall sexual function, mood, and libido; increases bone mineral density, energy, and lean body muscle mass; and decreases body fat mass.2

A Saliva Testosterone Test Measures Your Free Testosterone Level

As reviewed in our last blog post, both high and low free testosterone levels can cause health concerns; therefore, not everyone needs to increase their testosterone level. This is why it is important to test your saliva hormone levels and speak with your doctor about your test results.

Assessing your bioactive, free hormone levels is one benefit of saliva hormone testing; read about many other benefits in the blog post The Benefits of Saliva Hormone Testing.

Since saliva hormone testing offers an opportunity to directly test your free testosterone level, ordering a salivary testosterone level will provide insight into the level of testosterone that is actively affecting cells, tissues, and organs throughout your body.

Doctor Discussing a Salivary (Free) Testosterone Level with a Female Patient

What is Free Testosterone?

Free testosterone is the bioavailable testosterone that is actively affecting cells, tissues, and organs throughout your body.

The testosterone circulating in your blood is primarily bound to blood proteins that inactivate it. Most circulating testosterone predominantly binds to sex hormone-binding globulin (SHBG) and albumin, but small amounts also bind to corticosteroid-binding globulin (CBG) and alpha-1-acid glycoprotein (orosomucoid).3

Overall, only 2%–4% of your circulating testosterone is free, and the amount can differ significantly depending on many variables, including the levels of SHBG, albumin, CBG, and alpha-1-acid glycoprotein present in your blood.3 The binding proteins in the blood are large molecules and therefore do not filter into your saliva.

Testosterone bound to these large blood proteins is not active, which is why it is ideal to measure your free testosterone level in your saliva. Two individuals with the same total testosterone level in their blood can have significantly different active, free testosterone levels.

Young Active Healthy Women Comparing their Free Testosterone Levels

The Influence of Your Sex Hormone-Binding Globulin (SHBG) Level and Other Binding Protein Levels on Free Testosterone

The overall health of your liver, thyroid, kidneys, gastrointestinal tract, and other organs has a direct impact on your blood protein levels. Since most inactive testosterone is bound to sex hormone-binding globulin (SHBG), changes in the SHBG level in your blood will significantly affect your free testosterone level. Several specific health conditions are known to shift your SHBG levels. Obesity, acromegaly, diabetes mellitus, hypothyroidism, nephrotic syndrome, and metabolic syndrome are some of the conditions that could lower SHBG levels.3

Aging, weight loss, the use of estrogens, alcohol consumption, hepatitis, cirrhosis, and hyperthyroidism are associated with increased SHBG levels. Polymorphisms in the SHBG gene can also be an underlying cause of elevated or decreased SHBG levels.3

It is also interesting to note the overall fraction of circulating testosterone bound to SHBG varies in men and women. Approximately 44% of total testosterone is bound to SHBG in men, and 66% is bound to SHBG in women, so testing free salivary testosterone levels is crucial in both sexes.3

The binding of testosterone to albumin is quite complex. Testosterone competes with free fatty acids and certain medications, including ibuprofen and Coumadin, for binding sites on albumin. Therefore, albumin-binding medications and fluctuating free fatty acid levels in the blood could alter your free testosterone levels.3

The intricacies of testosterone binding to CBG and alpha-1-acid glycoprotein are not yet clear, but changes in your blood levels of albumin, CBG, and alpha-1-acid glycoprotein will alter your free testosterone level.3

Healthy Active Elderly Couple with Optimal Free Testosterone Levels Hiking Outside

Free Testosterone Levels by Age

Measuring free hormone levels becomes more important with aging due to many factors. Some postmenopausal women experience a significant reduction in sex hormone-binding globulin (SHBG) production, leading to elevated free testosterone levels and symptoms of hyperandrogenism.4 The signs and symptoms of hyperandrogenism will be reviewed in our next blog post.

In a well-designed study of women aged 65-74, the overall total blood testosterone level, including both free and protein-bound testosterone, was one-third the level observed in women in their 20s. But, the free testosterone level in the study population decreased by approximately 90%.5 Therefore, the total testosterone level could appear normal in women in this age range while their free testosterone level is clinically low.

Overall, free testosterone levels tend to decrease more significantly than total testosterone levels in older men and women.4 This reduces the biologically active pool of testosterone and increases the need for the direct measurement of free testosterone levels via a saliva hormone test.

Seniors with Healthy Free Salivary Testosterone Levels Hiking

Signs and Symptoms of a Low Free Testosterone Level (Testosterone Deficiency or Hypogonadism)

Men with low free testosterone levels may experience:

  • Low energy levels
  • Decreased lean muscle mass
  • Decreased libido
  • Metabolic syndrome
  • Anger & irritability
  • Erectile dysfunction
  • Infertility
  • Decreased physical stamina
  • Low Quality of Life
  • Diminished sense of well-being
  • Memory loss
  • Cognitive impairment
  • Increased fat mass and weight gain
  • Bone loss
  • Diabetes
  • Abnormal cholesterol levels
  • Insulin resistance
  • Atherosclerosis
  • Cardiac arrhythmia
  • Hypertension
  • Increased risk of cardiovascular disease2,6-8

Women with low free testosterone levels may experience:

  • Low energy levels
  • Decreased lean muscle mass
  • Sarcopenia
  • Decreased libido and hypo-active sexual desire disorder (HSDD)
  • Infertility
  • Mental disorders
  • Decreased physical stamina
  • Bone loss
  • Increased risk of bone fracture
  • Memory loss
  • Cognitive impairment
  • Cardiovascular disease5

Healthy Young Man and Young Woman with Optimal Free Testosterone Levels Hiking in Hawaii

How to Naturally Boost Your Testosterone Level

If you have signs or symptoms of low testosterone levels, consider having a conversation with your doctor about saliva hormone testing and what supportive natural protocol might be best for you based on your test results, family history, symptoms, and other factors.

If your free testosterone level is low, and your doctor suggests a natural protocol to boost T production, the protocol might include adrenal support, vitamins, minerals, increased strength training and other lifestyle changes, dietary changes, and botanicals that have improved testosterone levels in research studies. Evidence suggests botanicals such as Ashwagandha, bioflavonoids, including quercetin and chrysin, Tribulus terrestris L., Zingiber officinale (Ginger), Panax ginseng (Ginseng), Eurycoma longifolia (Tongkat Ali), and fenugreek seed extracts may support optimal testosterone levels.9-15

 Ginger Supports an Optimal Testosterone Level

Ginger and Testosterone

Research that links ginger supplementation to healthy testosterone levels demonstrates that ginger reduces oxidative stress and lipid peroxidation in the testes, enhances luteinizing hormone (LH) production by the pituitary gland in the brain, which stimulates testosterone production, increases blood flow in the testes, increases the level of cholesterol in the testes to support steroid hormone production, enhances the activity of antioxidant enzymes, increases testicular weight, normalizes blood glucose, and recycles testosterone receptors.11

Ashwagandha Supports a Healthy Free Testosterone Level

Ashwagandha and Testosterone

Testosterone deficiency is associated with metabolic syndrome, obesity, increased waist circumference, type 2 diabetes, high insulin levels, and poor overall health; therefore, optimizing weight by decreasing body fat percentage and increasing lean muscle mass with resistance training has been shown to increase testosterone levels.16,17

Research also shows stress reduction via stress management techniques supports optimal testosterone levels. Increasing the duration of sleep is also associated with improved serum testosterone levels. In 2011, Leproult et al. discovered sleep deprivation significantly decreased testosterone levels by 10% to 15%. Participants in the study restricted sleep to only 5 hours per night. Therefore, improving sleep hygiene and increasing the duration of quality sleep to 7-8 hours per night may increase natural testosterone production.18 Supporting optimal sleep, ideal body composition, and a healthy stress response are three mechanisms by which Ashwagandha supports natural testosterone production.19,20

 Strong Man Extending a Hand to Help His Friend While Hiking - Optimal Free Testosterone Level

Nutrients that may Boost Testosterone Production

Other nutrients that support increased testosterone production include L-carnitine, CoQ10, vitamin D, full-spectrum vitamin E, vitamin C, lipoic acid, selenium, zinc, boron, and magnesium.21-30

Healthy Young Couple with Optimal Salivary Testosterone Levels Cooking Together

Sex Hormone-Binding Globulin (SHBG) and the Free Testosterone Level

To directly increase your bioactive free testosterone level, consider strategies to decrease the Sex Hormone-Binding Globulin (SHBG) level in your blood. The use of oral estrogens, such as hormonal birth control pills in women, increases the SHBG level and reduces the free testosterone level.31

Unintentional exposure to bioidentical transdermal estrogens could also potentially increase the SHBG level in men because high SHBG levels are associated with higher estrogen levels in men.32,33

Salivary estrone (E1), estradiol (E2), and estriol (E3) tests can be ordered to rule out unintentional exposure to “hidden” estrogens in personal care products and other sources. Screening for unintentional exposure to bioidentical hormones is one benefit of saliva hormone testing, and avoiding estrogens can reduce your SHBG level.

Other natural options that might decrease SHBG levels include Urtica dioica root (stinging nettle root), caffeine avoidance, increased protein intake, alcohol avoidance, and Eurycoma longifolia (Tongkat Ali).34-38

Active Happy Woman and Friend Hiking to Boost Free Testosterone Level

Summary of Evidence-Based Natural Options that Support Healthy Free Testosterone Levels:

  • Weight and body composition optimization - decrease body fat percentage and increase lean muscle mass
  • Exercise (primarily resistance training)
  • Decrease stress
  • Increase quality sleep
  • Withania somnifera (Ashwagandha)
  • Ginger
  • Magnesium
  • Boron
  • Selenium
  • Zinc
  • L-Carnitine
  • CoQ10
  • Full Spectrum Vitamin E
  • Vitamin C
  • Alpha-lipoic acid
  • Tribulus terrestris
  • Panax ginseng
  • Decrease your Sex Hormone-Binding Globulin (SHBG) level
    • Avoid the use of oral estrogens in women and unintentional exposure to transdermal estrogens in men. Saliva hormone testing will screen for unintentional exposure to estrogens in men.
    • Urtica dioica root (Stinging Nettle root)
    • Increase protein intake
    • Avoid Caffeine
    • Avoid Alcohol
    • Eurycoma longifolia (Tongkat Ali)9-38
Zinc Plus contains zinc ascorbate, which supports optimal testosterone production

Zinc Plus contains zinc ascorbate, which supports optimal testosterone production.*

B-KalmPlexus contains Ashwagandha to support testosterone production, ideal body composition, and a healthy stress response.
Adapt contains Ashwagandha to support testosterone production, ideal body composition, and a healthy stress response.

B-KalmPlexus and Adapt contain Ashwagandha to support testosterone production, ideal body composition, and a healthy stress response.*

* This statement has not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure or prevent any disease.




Testosterone is considered the “elixir of life” and has been sought after for its rejuvenating properties for millennia. Testicular extracts from animals were used by the ancient Chinese and the Romans for men’s health. The idea and practice of optimizing hormone levels for health benefits became more widespread when the acclaimed scientist and endocrinologist Dr. Charles Brown-Séquard regularly injected himself with testicular extracts in the late 1800s to restore vitality.


Cortisol is known as the stress hormone because stress significantly affects the amount of cortisol released by your adrenal glands. Acute stress tends to increase your cortisol level, but cortisol is also produced during non-stressful times and crucial for the optimal function of your body every day. In healthy individuals, cortisol levels naturally shift throughout the day in a pattern known as a diurnal rhythm. A diurnal rhythm is a circadian rhythm that is repeated every 24 hours and synchronized with the day/night cycle.


April is Stress Awareness Month, so let’s learn more about the stress hormone known as cortisol! While cortisol is known as the stress hormone, it is crucial for the optimal function of your body every day. In healthy individuals, cortisol levels naturally shift throughout the day in a pattern known as a diurnal rhythm.


If you could choose, would you rather spit into a tube or have a needle jabbed into your arm to measure your hormone levels? We suspect you would rather not get stuck with a needle, and you do have a choice! Saliva hormone testing offers many benefits, including painless collection in the comfort of your home at any time. Saliva hormone tests can help determine the underlying cause(s) of PMS, insomnia, anxiety, fatigue, infertility, migraines, weight gain, hot flashes, hair loss, and many other health concerns.


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